Recombinant Human Suppressor Of Cytokine Signaling 3, Myc-DDK-tagged

• Cat.No.: SOCS3-6940H
• Product Overview: RecombinantHuman SOCS3 protein was expressed in HEK293 cells with Myc-DDKtag. MW: 24.6 kDa.

Specification

• Cat. No.: SOCS3-6940H
• Description: Thisgene encodes a member of the suppressor of cytokine signaling, family. SSIfamily members are cytokine-inducible negative regulators of cytokinesignaling. The expression of this gene is induced by various cytokines,including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by thisgene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase.
• Source: Human HEK293 cells
• Species: Human
• Molecular Weight: 24.6kDa
• Purity: > 80% asdetermined by SDS-PAGE and Coomassie blue staining.
• Preparation: Transfectedcells were cultured for 48-72 hrs before collection. The cells were lysed inmodified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA,1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), andthen centrifuged to clarify the lysate.
• Concentration: > 50ug/ml as determined by BCA.
• Endotoxin: < 0.1 EUper 1 μg of the protein by the LAL
• Reconstitution: 10%glycerol, 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles. Stable for 3months from receipt of products under proper storage and handling conditions.
• Tag: Myc&DDK

Gene Information

• Gene Name: SOCS3 suppressor ofcytokine signaling 3 [ Homo sapiens ]
• Official Symbol: SOCS3
• Synonyms: SOCS3;suppressor of cytokine signaling 3; CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3;CIS-3; STAT-induced STAT inhibitor 3; cytokine-induced SH2 protein 3; cytokine-inducibleSH2 protein 3; MGC71791
• Gene ID: 9021
• mRNA Refseq: NM_003955
• Protein Refseq: NP_003946
• MIM: 604176
• UniProt ID: P35270
• Chromosome Location: 2p14-p12
• Pathway: ATF-2 transcription factor network;Adaptive Immune System; Adipocytokine signaling pathway; Adipogenesis; ClassI MHC mediated antigen processing & presentation; Cytokine Signaling inImmune system; ECS complex; EGFR1 Signaling Pathway; EPO signaling pathway;Growth hormone receptor signaling; Hepatitis C; Herpes simplex infection;IL-2 Signaling Pathway; IL-3 Signaling Pathway; IL-4 signaling Pathway; IL-6Signaling Pathway
• Function: protein kinase inhibitor activity

Reviews

11/30/2022

SOCS3 is very stable in Western blot, and its performance will not be affected even if it is stored for a long time.

03/22/2022

SOCS3 has long-lasting effects and sustained release performance.

08/28/2020

This synthesis cost and application cost are moderate, and it is cost-effective for production.

Q&As

What are the treatment strategies for SOCS3?

Therapeutic strategies for SOCS3 can include regulating its expression level, blocking its interaction with other proteins, or regulating its associated signal transduction pathways. These approaches may have potential applications in the treatment of autoimmune diseases, inflammatory diseases, and certain cancers.

How about the relationship between the structure and function of SOCS3?

The structure of SOCS3 determines its ability and stability to bind to other proteins. The study of its structure can help us understand its function and mechanism of action.

What is the specific role of SOCS3 in inflammatory diseases?

In inflammatory diseases, SOCS3 may reduce the inflammatory response by inhibiting the production and release of pro-inflammatory cytokines and promoting the expression of anti-inflammatory factors. It could be a potential target for the treatment of inflammatory diseases.

Does there have interactions of SOCS3 with other proteins?

This protein can interact with a variety of other proteins, including JAK kinase, STAT transcription factors, etc. These interactions enable it to regulate processes such as cell growth, differentiation, and apoptosis.

What factors should be considered in drug development for SOCS3?

Drug development for SOCS3 requires consideration of its specificity, safety, pharmacokinetic properties, and potential side effects. In addition, issues such as the dosage form, route of administration and design of clinical trials need to be considered.

What is the main function of SOCS3 in the immune system?

SOCS3 is primarily involved in immunosuppressive and anti-inflammatory responses in the immune system. It can inhibit the production and release of pro-inflammatory cytokines and promote the expression of anti-inflammatory factors, thereby reducing the inflammatory response.