TNFRSF1A-1908H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Human Tumor Necrosis Factor Receptor Inhibitor produced inNSOis a disulfide linked homodimer, glycosylated, polypeptide chain. rHusTNFRI/Fc chimera (sTNFRI/Fc chimera) was fused to carboxy-terminal 6X his-tagged Fc part of human IgG1, via a linker peptide.
Description :
Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNF-alpha are mediated. TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses. It regulates immune and inflammatory responses.
Source :
Mammalian cells
Amino Acid Sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser.
Molecular Weight :
The predicted molecular weight of Recombinant Human TNF sRI is Mr 48 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 55-60 kDa.
Form :
Purified.
Purity :
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver-Stained gel.
Dimers & Aggregates :
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin :
Less than 0.1 ng/µg (IEU/µg) of rHusTNFRI.
Protein Content :
Protein quantitation was carried out by two independent methods: 1) UV spectroscopy at 280 nm. 2) Analysis by RP-HPLC, using a standard solution of rHusTNFRI as a Reference Standard.
Biological Activity :
rHusTNFRI is fully biologically active when compared to standards.The activity was measured by its ability to neutralize apoptosis of mouse L929 cells treated with 0.25 ng/mL rhTNF-a. The ED50 for this effect is typically 0.4-1 ng/mL.
Storage :
-20°C (aliquot), avoid repeated freeze and thaw cycles.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.