| Species : |
Human |
| Source : |
Hordeum Vulgare |
| Tag : |
HQ |
| Description : |
Tumor necrosis factor-alphais the best-known member of this class, and sometimes referred to when theterm tumor necrosis factor is used. TNF is a monocyte-derived cytotoxin thathas been implicated in tumour regression, septic shock and cachexia. |
| Form : |
Lyophilized from a0.2 μm sterile filtered solution of PBS, pH 7.2. |
| Purity : |
>98% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction. |
| Molecular Weight : |
Projected MW 19.6kDa. The recombinant protein migrates as two bands with an apparent MW 21 and23 kDa in SDS-PAGE. |
| Endotoxin Level : |
< 0.005 ng per μgof product (0.05 EU/μg) as measured by turbidimetric kinetic assay. |
| Reconstitution : |
Centrifuge vialprior to opening. First add sterile distilled water to the vial to fullysolubilize the protein to a concentration not less than 100 μg/ml. Aftercomplete solubilization of the protein, it can be further diluted to otheraqueous solutions. |
| Biological Activity : |
The ED50is determined by the Cytotoxicity Assay for the human-TNF-alpha fordosedependent cytolytic effects of serial dilutions of h-TNF-alpha on thefibrosarcoma L-929 cells. The ED50 for a typical batch ofrecombinant human TNF-alpha is 40 ng/ml. |
| Storage : |
The lyophilizedprotein, though stable at room temperature for two weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| OfficialSymbol : |
TNF |
| Pathways : |
Adipocytokinesignaling pathway; Adipogenesis; African trypanosomiasis; Allograftrejection; Alzheimer" s disease; Amoebiasis; Amyotrophic lateral sclerosis(ALS); Angiopoietin receptor Tie2-mediated signaling; Antigen processing andpresentation; Apoptosis; Asthma; Calcineurin-regulated NFAT-dependenttranscription in lymphocytes; Canonical NF-kappaB pathway; Caspase cascade inapoptosis; Cellular roles of Anthrax toxin; Ceramide signaling pathway |
