| Description : |
The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15. |
| Source : |
Baculovirus-Insect Cells |
| Species : |
Human |
| Tag : |
N-His |
| Molecular Mass : |
The protein has a calculated MW of 43.8 kDa. |
| AA Sequence : |
MHHHHHHSKAFGLLRQICQSILAES SQSPADLEEKKEEDSNMKREQPRER PRAWDYPHGLVGLHNIGQTCCLNSL IQVFVMNVDFTRILKRITVPRGADE QRRSVPFQMLLLLEKMQDSRQKAVR PLELAYCLQKCNVPLFVQHDAAQLY LKLWNLIKDQITDVHLVERLQALYT IRVKDSLICVDCAMESSRNSSMLTL PLSLFDVDSKPLKTLEDALHCFFQP RELSSKSKCFCENCGKKTRGKQVLK LTHLPQTLTIHLMRFSIRNSQTRKI CHSLYFPQSLDFSQILPMKRESCDA EEQSGGQYELFAVIAHVGMADSGHY CVYIRNAVDGKWFCFNDSNICLVSW EDIQCTYGNPNYHWQETAYLLVYMK MEC |
| Purity : |
> 90% by SDS-PAGE |
| Storage : |
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Concentration : |
0.377 mg/mL |
| Storage Buffer : |
50 mM Hepes, 200 mM NaCl, pH 7.5, 2 mM DTT, 0.5 mM EDTA, 10% Glycerol |
