Active Recombinant Mouse Usp18 Protein, His-tagged

Cat.No. : Usp18-32M
Product Overview : Recombinant mouse Usp18 protein (Ser47 - Ser368) contains an N-terminal 6-His tag was expressed in Spodoptera frugiperda, Sf 21 (baculovirus).
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Description : Involved in the regulation of inflammatory response to interferon type 1. Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.
Source : Insect cell
Species : Human
Tag : His
Bio-activity : Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant murine USP18 concentration of 100-250 nM when using ISG15-AMC as a substrate.
Molecular Mass : 38 kDa
Purity : >98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
24 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
Storage Buffer : X mg/mL (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 5 mM DTT and 1 mM EDTA
Gene Name : Usp18 ubiquitin specific peptidase 18 [ Mus musculus (house mouse) ]
Official Symbol : Usp18
Synonyms : 43 kDa ISG15-specific protease; ISG15-specific-processing protease; ubiquitin specific protease 15; ubiquitin specific protease 18; ubl thioesterase 18; ubl thiolesterase 18; EC 3.1.2.-; Usp18; ubiquitin specific peptidase 18; UBP43; Ubp15; AW047653; 1110058H21Rik
Gene ID : 24110
mRNA Refseq : NM_011909
Protein Refseq : NP_036039
UniProt ID : Q9WTV6

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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