Recombinant Human WHSC2, His-tagged

• Cat.No.: WHSC2-30860TH
• Product Overview: Recombinant full length Human WHSC2 (amino acids 1-539) with an N terminal His tag; 569 amino acids with tag, Predicted MWt 60.6kDa,

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 539 amino acids
• Description: This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.
• Conjugation: HIS
• Molecular Weight: 60.600kDa inclusive of tags
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 1.17% Sodium chloride, 0.08% DTT, 0.03% EDTA, 20% Glycerol
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMPGQRRALSPKMASMRESDT GLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLS SAVKLKLLLGTLHLPRRTVDEMKGALMEIIQLASLDSDPW VLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVG ECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKP KSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMD TTTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLR KERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKE ETVVENATPDYAAGLVSTQKLGSLNNEPALPSTSYLPSTP SVVPASSYIPSSETPPAPSSREASRPPEEPSAPSPTLPAQ FKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQT PPVAMVAPQTQAPAQQQPKKNLSLTREQMFAAQEMFKTAN KVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDL PKADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS

Gene Information

• Gene Name: WHSC2 Wolf-Hirschhorn syndrome candidate 2 [ Homo sapiens ]
• Official Symbol: WHSC2
• Synonyms: WHSC2; Wolf-Hirschhorn syndrome candidate 2; negative elongation factor A; NELF A
• Gene ID: 7469
• mRNA Refseq: NM_005663
• Protein Refseq: NP_005654
• MIM: 606026
• Uniprot ID: Q9H3P2
• Chromosome Location: 4p16.3
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Formation and Maturation of mRNA Transcript, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem; Formation of RNA Pol II elongation complex, organism-specific biosystem