Recombinant Mouse Adprhl2 Protein, MYC/DDK-tagged

• Cat.No.: Adprhl2-022M
• Product Overview: Purified recombinant protein of full-length mouse ADP-ribosylhydrolase like 2 (Adprhl2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: ADP-ribose glycohydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose. Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds. Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos. Also hydrolyzes free poly(ADP-ribose) in mitochondria. Specifically digests O-acetyl-ADP-D-ribose, a product of deacetylation reactions catalyzed by sirtuins. Specifically degrades 1''-O-acetyl-ADP-D-ribose isomer, rather than 2''-O-acetyl-ADP-D-ribose or 3''-O-acetyl-ADP-D-ribose isomers.
• Molecular Mass: 39.9 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Adprhl2 ADP-ribosylhydrolase like 2 [ Mus musculus (house mouse) ]
• Official Symbol: Adprhl2
• Synonyms: Adprhl2; ADP-ribosylhydrolase like 2; Arh3; Adprs; AI836109; ADP-ribose glycohydrolase ARH3; ADP-ribosylhydrolase 2; ADP-ribosylhydrolase 3; O-acetyl-ADP-ribose deacetylase ARH3; [Protein ADP-ribosylarginine] hydrolase-like protein 2; [Protein ADP-ribosylserine] hydrolase; ecto ADP-ribosylhydrolase; poly(ADP-ribose) glycohydrolase ARH3; EC 3.2.1.143
• Gene ID: 100206
• mRNA Refseq: NM_133883
• Protein Refseq: NP_598644
• UniProt ID: Q8CG72