Recombinant Mouse Cpsf6 Protein, Myc/DDK-tagged

• Cat.No.: Cpsf6-2297M
• Product Overview: Purified recombinant protein of mouse full-length cleavage and polyadenylation specific factor 6 (Cpsf6), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery. Plays a role in mRNA export.
• Molecular Mass: 59.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cpsf6 cleavage and polyadenylation specific factor 6 [ Mus musculus (house mouse) ]
• Official Symbol: Cpsf6
• Synonyms: CPSF6; cleavage and polyadenylation specific factor 6; cleavage and polyadenylation specificity factor subunit 6; CFIM; CFIM68; AI256641; HPBRII-4; HPBRII-7; 4733401N12Rik
• Gene ID: 432508
• mRNA Refseq: NM_001013391
• Protein Refseq: NP_001013409
• UniProt ID: Q6NVF9