Recombinant Mouse Dnajb12 Protein, Myc/DDK-tagged

• Cat.No.: Dnajb12-2596M
• Product Overview: Purified recombinant protein of mouse full-length DnaJ heat shock protein family (Hsp40) member B12 (Dnajb12), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway. Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities. Can also act independently of HSPA8/Hsc70: together with DNAJB14, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers. While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70. When overexpressed, forms membranous structures together with DNAJB14 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear.
• Molecular Mass: 42 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dnajb12 DnaJ heat shock protein family (Hsp40) member B12 [ Mus musculus (house mouse) ]
• Official Symbol: Dnajb12
• Synonyms: Dnajb12; DnaJ heat shock protein family (Hsp40) member B12; Dj10; mDj10; dnaJ homolog subfamily B member 12; DnaJ (Hsp40) homolog, subfamily B, member 12; DnaJ homolog 10
• Gene ID: 56709
• mRNA Refseq: NM_019965
• Protein Refseq: NP_064349
• UniProt ID: Q9QYI4