| Description : |
Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares homology with KGF, and both KGF and FGF-10 activate the receptor FGFR2-IIIb. However, unlike KGF, which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during multi-organ development via mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development and is regulated by Shh during early development. |
| Source : |
E. coli |
| Species : |
Mouse |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 10 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 1.0 × 10^5 units/mg. |
| Molecular Mass : |
17.0 kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
148 |
| AA Sequence : |
SSAGRHVRSYNHLQGDVRWRRLFSF TKYFLTIEKNGKVSGTKNEDCPYSV LEITSVEIGVVAVKAINSNYYLAMN KKGKLYGSKEFNNDCKLKERIEENG YNTYASFNWQHNGRQMYVALNGKGA PRRGQKTRRKNTSAHFLPMTIQT |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant mouse Fibroblast Growth Factor-10 (rmFGF-10) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rmFGF-10 remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O at 100 μg/mL. |
