Active Recombinant Mouse FGF12 Protein

• Cat.No.: FGF12-33M
• Product Overview: Recombinant Mouse FGF12 Protein (Met1-Thr181) is produced by E. coli expression system.

Specification

• Source: E. coli
• Species: Mouse
• Form: 0.22 µm filtered protein solution is in 20 mM MOPS, 150 mM Na2SO4, 1 mM EDTA, 10 mM DTT, pH 7.5.
• Bio-activity: The activity was measured by the binding to human FGFR4-Fc using functional ELISA. The ED50 is 0.01-0.05 µg/ml when human FGFR4-Fc is immobilized at 5 µg/ml (100 µl/ well)
• Molecular Mass: The 181 amino acid recombinant protein has a predicted molecular mass of approximately 21 kD. The DTT-reduced protein migrates at approximately 21 by SDS-PAGE. The predicted N-terminal amino acid is Met.
• Protein length: Met1-Thr181
• Endotoxin: < 0.1 EU/µg cytokine as determined by the LAL method.
• Purity: > 98%, as determined by Coomassie stained SDS-PAGE.
• Applications: Bioassay
• Storage: Unopened vial can be stored between 2 centigrade and 8 centigrade for one month, at -20 centigrade for six months, or at -70 centigrade for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 centigrade to -70 centigrade. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Concentration: 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial.

Gene Information

• Gene Name: Fgf12 fibroblast growth factor 12 [ Mus musculus ]
• Official Symbol: FGF12
• Synonyms: FGF12; fibroblast growth factor 12; FHF-1; FGF-12; Fhf1; AV114868
• Gene ID: 14167
• mRNA Refseq: NM_010199
• Protein Refseq: NP_034329