Recombinant Human FGF12, His-tagged

• Cat.No.: FGF12-26554TH
• Product Overview: Recombinant full length Human FGF12 isoform 2 with a N terminal His tag. 201 amino acids with a predicted MWt 22.6 kDa including tag.

Specification

• Description: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Protein length: 181 amino acids
• Conjugation: HIS
• Molecular Weight: 22.600kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Brain, eye and testis; highly expressed in embryonic retina, olfactory epithelium, olfactory bulb, and in a segmental pattern of the body wall; in adult olfactory bulb, less in cerebellum, deep cerebellar nuclei, cortex and multiple midbrain structures.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Constituents:0.32% Tris HCl, 0.02% DTT, 0.06% EDTA, 10% Glycerol
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MSSHHHHHH SSGLVPRGSH MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE YLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREQS LHEIGEKQGR RKSSGTPTM NGGKVVNQDS T
• Sequence Similarities: Belongs to the heparin-binding growth factors family.

Gene Information

• Gene Name: FGF12 fibroblast growth factor 12 [ Homo sapiens ]
• Official Symbol: FGF12
• Synonyms: FGF12; fibroblast growth factor 12; FGF12B; FHF1; fibroblast growth factor 12B; fibroblast growth factor FGF 12b; fibroblast growth factor homologous factor 1; myocyte activating factor
• Gene ID: 2257
• mRNA Refseq: NM_004113
• Protein Refseq: NP_004104
• MIM: 601513
• Uniprot ID: P61328
• Chromosome Location: 3q28
• Pathway: MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; Melanoma, organism-specific biosystem; Melanoma, conserved biosystem; Pathways in cancer, organism-specific biosystem
• Function: NOT fibroblast growth factor receptor binding; growth factor activity; heparin binding