Recombinant Mouse Hnrnpu Protein, Myc/DDK-tagged
Cat.No. : | Hnrnpu-3422M |
Product Overview : | Purified recombinant protein of mouse full-length heterogeneous nuclear ribonucleoprotein U (Hnrnpu), with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
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Species : | Mouse |
Source : | HEK293 |
Tag : | DDK&Myc |
Description : | DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression. Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability. Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation. Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator. Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation. Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus. Negatively regulates glucocorticoid-mediated transcriptional activation. Key regulator of transcription initiation and elongation in embryonic stem cells upon leukemia inhibitory factor (LIF) signaling. Involved in the long non-coding RNA H19-mediated Pol II transcriptional repression. Participates in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. Plays a role in the regulation of telomere length. Plays a role as a global pre-mRNA alternative splicing modulator by regulating U2 small nuclear ribonucleoprotein (snRNP) biogenesis. Plays a role in mRNA stability. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Enhances the expression of specific genes, such as tumor necrosis factor TNFA, by regulating mRNA stability, possibly through binding to the 3'-untranslated region (UTR). Plays a role in mitotic cell cycle regulation. Involved in the formation of stable mitotic spindle microtubules (MTs) attachment to kinetochore, spindle organization and chromosome congression. Phosphorylation at Ser-58 by PLK1 is required for chromosome alignement and segregation and progression through mitosis. Contributes also to the targeting of AURKA to mitotic spindle MTs. Binds to double- and single-stranded DNA and RNA, poly(A), poly(C) and poly(G) oligoribonucleotides. Binds to chromatin-associated RNAs (caRNAs). Associates with chromatin to scaffold/matrix attachment region (S/MAR) elements in a chromatin-associated RNAs (caRNAs)-dependent manner. Binds (via RNA-binding RGG-box region) to the long non-coding Xist RNA; this binding is direct and bridges the Xist RNA and the inactive chromosome X (Xi). Binds the long non-coding H19 RNA. Binds to SMN1/2 pre-mRNAs at G/U-rich regions. Binds to small nuclear RNAs (snRNAs). Binds to the 3'-UTR of TNFA mRNA. Also negatively regulates embryonic stem cell differentiation upon LIF signaling. Required for embryonic development. Binds to brown fat long non-coding RNA 1 (Blnc1); facilitates the recruitment of Blnc1 by ZBTB7B required to drive brown and beige fat development and thermogenesis. |
Molecular Mass : | 88.4 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 μg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name | Hnrnpu heterogeneous nuclear ribonucleoprotein U [ Mus musculus (house mouse) ] |
Official Symbol | Hnrnpu |
Synonyms | HNRNPU; heterogeneous nuclear ribonucleoprotein U; SAF-A; nuclear matrix protein sp120; scaffold attachment factor A; heterogenous nuclear ribonucleoprotein U; SAFA; Hnrpu; Sp120; C86794; hnRNP U; AA408410; AI256620; AL024194; AL024437; AW557595 |
Gene ID | 51810 |
mRNA Refseq | NM_016805 |
Protein Refseq | NP_058085 |
UniProt ID | Q8VEK3 |
◆ Recombinant Proteins | ||
HNRNPU-4381H | Recombinant Human HNRNPU Protein, Myc/DDK-tagged, C13 and N15-labeled | +Inquiry |
HNRNPU-4907H | Recombinant Human HNRNPU Protein, GST-tagged | +Inquiry |
HNRNPU-3654HF | Recombinant Full Length Human HNRNPU Protein, GST-tagged | +Inquiry |
HNRNPU-7769M | Recombinant Mouse HNRNPU Protein | +Inquiry |
Hnrnpu-3422M | Recombinant Mouse Hnrnpu Protein, Myc/DDK-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
HNRNPU-5439HCL | Recombinant Human HNRNPU 293 Cell Lysate | +Inquiry |
Not For Human Consumption!
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