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Recombinant Mouse Il18r1 protein, His&hFc-tagged

Cat.No. : Il18r1-3319M
Product Overview : Recombinant Mouse Il18r1 protein(NP_032391.1)(Met1-Gly326), fused with His&hFc tag, was expressed in HEK293.
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Source : HEK293
Species : Mouse
Tag : His&hFc
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Mass : The recombinant mouse IL18R1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 555 amino acids and has a predicted molecular mass of 63.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm IL18R1/Fc monomer is approximately 95-105 kDa due to glycosylation.
Protein Length : Met1-Gly326
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 98 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Il18r1 interleukin 18 receptor 1 [ Mus musculus ]
Official Symbol : Il18r1
Synonyms : IL18R1; interleukin 18 receptor 1; interleukin-18 receptor 1; IL-18R1; IL-1Rrp; IL1R-rp; IL-18R-1; IL1 receptor-related protein; CD218 antigen-like family member A; interleukin 1 receptor related protein; Il1rrp; Il18ralpha;
Gene ID : 16182
mRNA Refseq : NM_001161842
Protein Refseq : NP_001155314

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How do researchers study the co-receptor interactions involving IL18R1 and IL18RAP, and what methods are employed to decipher their cooperative signaling effects? 09/05/2022

Co-immunoprecipitation, co-receptor binding assays, and functional studies unveil the cooperative roles of IL18R1 and IL18RAP in signaling.

How are imaging techniques, such as fluorescence microscopy and live-cell imaging, applied to visualize the dynamics of IL18R1 localization and clustering upon IL18 stimulation? 04/09/2021

Fluorescence microscopy, confocal imaging, and super-resolution microscopy visualize IL18R1 dynamics and clustering upon IL18 stimulation.

What experimental methods are employed to investigate the post-translational modifications of IL18R1 and their impact on receptor function and downstream signaling? 10/05/2020

Mass spectrometry, phosphorylation assays, and ubiquitination studies investigate IL18R1 post-translational modifications.

How do researchers experimentally explore the role of IL18R1 in disease contexts, and what techniques are used to assess its potential as a therapeutic target? 08/05/2019

Disease model assessments, patient sample analyses, and pharmacological interventions explore IL18R1's involvement in diseases and therapeutic potential.

Could you explain the strategies used to assess the expression levels and tissue distribution of IL18R1 in different cell types and under varying physiological conditions? 04/26/2019

RT-qPCR, flow cytometry, and single-cell RNA sequencing assess IL18R1 expression across different cell types and physiological states.

Can you provide insights into the use of knockout mice, knockdown approaches, and gene editing techniques to elucidate the physiological importance of IL18R1? 03/14/2019

Knockout models, siRNA-mediated knockdown, and CRISPR/Cas9 gene editing reveal the physiological roles of IL18R1 in vivo and in vitro.

What cellular assays and functional studies are commonly utilized to investigate the downstream signaling cascades activated by IL18R1 upon IL18 binding? 09/19/2018

Western blotting, ELISAs, and phosphoprotein analyses decipher IL18R1-mediated signaling cascades and cytokine production.

What experimental approaches are commonly used to dissect the role of IL18R1's extracellular and intracellular domains in mediating IL18-induced immune responses? 06/24/2018

Site-directed mutagenesis, chimera studies, and domain swapping experiments dissect the functional contributions of IL18R1's domains.

Can you elaborate on the techniques used to investigate the structural basis of IL18R1's interaction with IL18 and its implications for downstream signaling? 10/04/2017

X-ray crystallography, cryo-electron microscopy, and molecular dynamics simulations elucidate the structural basis of IL18R1-IL18 recognition and activation.

How do researchers experimentally validate the binding affinity and kinetics of IL18R1 to IL18, considering their central role in initiating IL18-mediated signaling pathways? 09/07/2017

Surface plasmon resonance, bio-layer interferometry, and kinetic binding assays quantify IL18R1-IL18 interactions.

Customer Reviews (3)

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Reviews
03/07/2020

    Low endotoxin levels.

    03/15/2018

      Good for flow cytometry.

      09/06/2017

        Potent in Th1 activation.

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