Recombinant Mouse Lep

• Cat.No.: Lep-226M

Specification

• Cat. No.: Lep-226M
• Description: Lep is a 16 kDa protein hormone that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. The Lep gene is located on chromosome 7 in humans.
• Source: E. coli
• Species: Mouse
• Form: Filtered (0, 4 μm) and lyophilized in 0.5 mg/mL in 10 mM sodium citrate, pH 4.0
• Research topic: Energy metabolism and body weight regulation
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Once the protein is solubilized, pH can be readjusted to the physiological range. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: ELISA; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: VPIQKVQDDT KTLIKTIVTR INDISHTQSV SAKQRVTGLD FIPGLHPILS LSKMDQTLAV YQQVLTSLPS QNVLQIANDL ENLRDLLHLL AFSKSCSLPQ TSGLQKPESL DGVLEASLYS TEVVALSRLQ GSLQDILQQL DVSPEC
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: Lep leptin [ Mus musculus ]
• Official Symbol: Lep
• Synonyms: Lep; Leptin; ob; obese; obesity factor
• Gene ID: 16946
• mRNA Refseq: NM_008493
• Protein Refseq: NP_032519
• UniProt ID: P41160
• Chromosome Location: 6
• Pathway: Adipocytokine signaling pathway; Adipogenesis; Cytokine-cytokine receptor interaction; Diabetes pathways; Incretin Synthesis; Integration of energy metabolism; Jak-STAT signaling pathway; Leptin and adiponectin; Neuroactive ligand-receptor interaction; Regulation of Insulin Secretion; Synthesis
• Function: growth factor activity; hormone activity; peptide hormone receptor binding; protein binding