| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Non |
| Description : |
Leptin, also known as obesity factor and obese protein, is a member of the adipocytokine family that are cytokines expressed from adipose tissues. Other members of the adipocytokine family include TNF alpha, IL-6 and resistin. The general function of these molecules is regulation of food intake energy homeostasis, hematopoiesis, inflammation and immunity. |
| Amino Acid Sequence : |
VPIQKVQDDTKTLI KTIVTRINDISHTQ SVSSKQKVT GLDFIPGLHP ILTLSK MDQT LAV YQQILTSMPSRNV IQISNDLENLRDLLH VLAFSKSCHLPWAS GLETL DSLGG VLEA SGYSTEVVALSRLQ GSLQDMLWQLDLSPGC. |
| Molecular Mass : |
Under reducing conditions Leptin migrates as a band at approximately 15-16 kDa in SDS-PAGE. This compares with unmodified Leptin that has a predicted monomeric molecular mass of 16 kDa. |
| PI : |
Leptinseparates into a number of isoforms with a pI between 5.0 and 7.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Leptin that has a predicted pI of 5.7. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
| Activity : |
The ED50of Leptin is typically 0.25-0.35 ng/ml as measured in a cytotoxicity assay using the human K562 cell line. |
