Recombinant Mouse Map3k5 Protein, Myc/DDK-tagged

• Cat.No.: Map3k5-3931M
• Product Overview: Purified recombinant protein of mouse full-length mitogen-activated protein kinase kinase kinase 5 (Map3k5), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 154.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Map3k5 mitogen-activated protein kinase kinase kinase 5 [ Mus musculus (house mouse) ]
• Official Symbol: Map3k5
• Synonyms: MAP3K5; mitogen-activated protein kinase kinase kinase 5; ASK-1; MEKK 5; MEK kinase 5; MAPK/ERK kinase kinase 5; apoptosis signal-regulating kinase 1; mitogen activated protein kinase kinase kinase 5; ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik; MGC141518; MGC141519
• Gene ID: 26408
• mRNA Refseq: NM_008580
• Protein Refseq: NP_032606
• UniProt ID: O35099