Recombinant Mouse Padi4 Protein, His-tagged

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Recombinant Mouse Padi4 Protein, His-tagged

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Cat.No. : Padi4-11M
Product Overview : Recombinant mouse Padi4 protein (2-666) with a N-terminal His-tag was expressed in insect cells.
Description : Protein arginine deiminase 4 (PAD4) is a murine peptidylarginine deiminase expressed in neutrophils, as well as brain, liver, lung, spleen, kidney, muscle, spinal cord, and testicular tissues. Upon activation of neutrophils, PAD4 translocates to the nucleus, where it catalyzes the citrullination of histone protein arginine residues. Neutrophils isolated from PAD4-/- mice exhibit decreased citrullination of histone H3 under both basal and LPS-stimulated conditions and are defective for the formation of neutrophil extracellular traps (NETs) in response to stimulation with LPS, phorbol 12-myristate 13-acetate, or hydrogen peroxide. PAD4-/- mice exhibit larger lesions than wild-type mice in a model of necrotizing fasciitis induced by M1 group A S. pyogenes lacking the extracellular DNase Sda1. PAD4-deficient mice also exhibit reduced infarct size in a model of myocardial ischemia-reperfusion injury and reduced tumor growth in a Lewis lung carcinoma model compared to wild-type controls.
Source : Insect cell
Species : Mouse
Tag : His
Form : 50 mM Tris HCl, pH 8.0, with 500 mM NaCl and 5% glycerol
Molecular Mass : 76.08 kDa
Protein length : 2-666
Purity : ≥90% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme to produce 1 nmol of NH4+ per minute at 37 centigrade in 50 mM HEPES, pH 7.7, containing 10 mM calcium chloride, 5 mM DTT, and 2 mM N-α-benzoyl-L-arginine ethyl ester (BAEE).
Applications : WB, ELISA, Enzymatic Assay
Stability : ≥ 1 year
Storage : Store at -80 centigrade
SDS-PAGE of Padi4 :

Lane 1: MW Markers, Lane 2: PAD4 (2 µg), Lane 3: PAD4 (4 µg). Representative gel image shown; actual purity may vary between each batch.

Activity Assay :

Gene Name : Padi4 peptidyl arginine deiminase, type IV [ Mus musculus (house mouse) ]
Official Symbol : Padi4
Synonyms : Padi4; peptidyl arginine deiminase, type IV; Pad4; Pdi4; protein-arginine deiminase type-4; PAD type IV; peptidylarginine deiminase IV; protein-arginine deiminase type IV
Gene ID : 18602
mRNA Refseq : NM_011061
Protein Refseq : NP_035191
UniProt ID : Q9Z183
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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