Recombinant Rat CD79B cell lysate
Cat.No. : | CD79B-1323RCL |
Product Overview : | Rat CD79B / B29 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Human cells |
Species : | Rat |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Tag : | Non |
Gene Name : | Cd79b Cd79b molecule, immunoglobulin-associated beta [ Rattus norvegicus ] |
Official Symbol : | CD79B |
Synonyms : | CD79B; Cd79b molecule, immunoglobulin-associated beta; B-cell antigen receptor complex-associated protein beta chain; CD79B antigen; immunoglobulin-associated beta; B29; Igb; MGC108607; |
Gene ID : | 171055 |
mRNA Refseq : | NM_133533 |
Protein Refseq : | NP_598217 |
Pathway : | B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewRenowned for its reliability and consistency, this protein offers researchers the confidence to achieve accurate and reproducible results in their studies.
The CD79B protein comes highly recommended for its outstanding performance in ELISA assays.
One notable advantage of the CD79B protein is the excellent technical support provided by its manufacturer.
Q&As (5)
Ask a questionCD79B-targeted immunotherapy aims to specifically target B cells, potentially reducing side effects compared to non-specific chemotherapy.
Analyzing CD79B expression helps tailor treatment plans, allowing for more personalized and effective approaches.
Yes, monitoring CD79B levels can aid in detecting minimal residual disease, providing insights into treatment response.
Variability in CD79B expression among different B cell disorders may pose challenges in its use as a standalone diagnostic marker.
Yes, several clinical trials are exploring the efficacy of CD79B-targeted drugs in various B cell disorders.
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