| Description : |
Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids. It contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). The polypeptide structure of CXC3L1 differs from the typical structure of other chemokines. For example, the spacing of the characteristic N-terminal cysteinesis different; there are three amino acids separating the initial pair of cysteines in CX3CL1, while there are none in CC chemokines and only one in CXC chemokines. CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk allows it to bind to the surface of certain cells. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 can signal through the chemokine receptor CX3CR1. |
| Source : |
HEK 293 |
| Species : |
Rat |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
The EC50 value of rat Fractalkine/CX3CL1 on Ca^2+ mobilization assay in CHO-K1/Gα15/rCX3CR1 cells (human Gα15 and rat CX3CR1 stably expressed in CHO-K1 cells) is less than 1 μg/mL. |
| Molecular Mass : |
70-90 kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
310 |
| AA Sequence : |
QHLGMTKCNITCHKMTSPIPVTLLI HYQLNQESCGKRAIILETRQHRHFC ADPKEKWVQDAMKHLDHQTAALTRN GGKFEKRVDNVTPRITSATRGLSPT ALAKPESATVEDLTLEPTAISQEAR RPMGTSQEPPAAVTGSSPSTSKAQD AGLAAKPQSTGISEVAAVSTTIWPS SAVYQSGSSLWAEEKATESPPTIAL STQASTTSSPKQNVGSEGQPPWVQE QDSTPEKSPGPEETNPVHTDIFQDR GPGSTVHPSVAPTSSEKTPSPELVA SGSQAPKVEEPIHATADPQKLSVFI TPVPDSQAAT |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 98% as analyzed by SDS-PAGE. |
| Storage : |
Lyophilized recombinant Rat Fractalkine/CX3CL1 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, recombinant Rat Fractalkine/CX3CL1 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
