| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
81-336 aa |
| Form : |
Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Bio-activity : |
Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) also known as fractalkine is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of CX3CL1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (150ul cell suspension,10^6 cells/ml in RPMI 1640 with FBS free) and CX3CL1 (0.1ng/ml, 0.01ng/ml,0.001ng/ml, 0.0001 and 0.00001ng/ml diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter(8um pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 1h, the filter was removed,then cells in low chamber were observed by inverted microscope at low magnification (×10) and the number of migrated cells were counted using Fluorescence Activating Cell Sorter. Result shows CX3CL1 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification(×10) were shown. Statistical results of FACS were shown. The optimum chemotaxis of CX3CL1 occurs at 0.001-0.00001ng/ml. |
| Molecular Mass : |
28.5kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 95% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
