Active Recombinant Rat Il10 Protein
Cat.No. : | Il10-260I |
Product Overview : | Recombinant Rat Il10 Protein without tag was expressed in CHO. |
- Specification
- Gene Information
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Description : | Interleukin-10 (IL-10), initially known as Cytokine Synthesis Inhibitory Factor (CSIF), belongs to the IL-10 family and shares more than 80% sequence homology with the Epstein-Barr Virus protein BCRFI. It is produced by many immune cells, such as T-cells, macrophages, mast cells and dendritic cells. It is usually secreted as a homodimer and, upon binding to its receptor, inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and Th2 cells. It also displays the ability to suppress Antigen-Presenting Cell (APC) function. The net effect of Interleukin-10 appears to be inhibitory; however, stimulatory effects, such as stimulation of B cell maturation and antibody production, are also reported. |
Source : | CHO |
Species : | Rat |
Form : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : | ED50 < 8μg/mL, measured in a bioassay using C6 cells. |
Molecular Mass : | 8-22 kDa, observed by reducing SDS-PAGE. |
AA Sequence : | SKGHSIRGDNNCTHFPVSQTHMLRE LRAAFSQVKTFFQKKDQLDNILLTD SLLQDFKGYLGCQALSEMIKFYLVE VMPQAENHGPEIKEHLNSLGEKLKT LWIQLRRCHRFLPCENKSKAVEQVK NDFNKLQDKGVYKAMNEFDIFINCI EAYVTLKMKN |
Endotoxin : | < 0.2 EU/μg, determined by LAL method. |
Purity : | > 95% as analyzed by SDS-PAGE and HPLC. |
Storage : | Lyophilized recombinant rat Interleukin-10(IL-10) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Rat Interleukin-10 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
Storage Buffer : | Lyophilized after extensive dialysis against PBS. |
Reconstitution : | Reconstituted in ddH2O or PBS at 100 μg/mL. |
Gene Name : | Il10 interleukin 10 [ Rattus norvegicus ] |
Official Symbol : | Il10 |
Synonyms : | IL10; interleukin 10; interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor; IL10X; |
Gene ID : | 25325 |
mRNA Refseq : | NM_012854 |
Protein Refseq : | NP_036986 |
UniProt ID : | P29456 |
Products Types
◆ Recombinant Protein | ||
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Il10-029I | Active Recombinant Mouse Il10 Protein (161 aa) | +Inquiry |
IL10-13H | Active Recombinant Human IL10 Protein, Pre-aliquoted | +Inquiry |
Il10-371I | Active Recombinant Rat Il10 Protein (161 aa) | +Inquiry |
IL10-134H | Recombinant Active Human IL10 Protein, His-tagged(C-ter) | +Inquiry |
◆ Lysates | ||
IL10-2079HCL | Recombinant Human IL10 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionCutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.
Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.
Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.
Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.
In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.
State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.
Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.
Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.
Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.
CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.
Customer Reviews (3)
Write a reviewEffective in anti-inflammatory assays.
High purity levels.
Stable under long-term storage.
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