Active Recombinant Rat Il10 protein

Species :	Rat
Source :	E.coli
Tag :	Non
Protein Length :	19-178 aa
Form :	Lyophilized from a 0.2 µm filtered solution in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl.
Bio-activity :	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10^6 IU/mg.
Molecular Mass :	Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.
AASequence :	SKGHSIRGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNILLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN
Endotoxin :	Less than 0.1 EU/µg of rRtIL-10 as determined by LAL method.
Purity :	> 95 % by SDS-PAGE analyse.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	Il10 interleukin 10 [ Rattus norvegicus ]
Official Symbol	Il10
Synonyms	IL10; interleukin 10; interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor; IL10X;
Gene ID	25325
mRNA Refseq	NM_012854
Protein Refseq	NP_036986