| Description : |
Factor involved in the inhibition of cytokine synthesis |
| Source : |
E.coli |
| Species : |
Rat |
| Form : |
Lyophilized from a 0.2μm filtered solution in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg. |
| Molecular Mass : |
Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids. |
| Protein length : |
160 |
| AA Sequence : |
SKGHSIRGDNNCTHFPVSQTHMLRE LRAAFSQVKTFFQKKDQLDNILLTD SLLQDFKGYLGCQALSEMIKFYLVE VMPQAENHGPEIKEHLNSLGEKLKT LWIQLRRCHRFLPCENKSKAVEQVK NDFNKLQDKGVYKAMNEFDIFINCI EAYVTLKMKN |
| Endotoxin : |
Less than 0.1 EU/µg of rRtIL-10 as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
