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Active Recombinant Rat Il3β Protein (144 aa)

Cat.No. : Il3-355I
Product Overview : Recombinant rat Interleukin-3 beta (rrIL-3 beta) produced in E. coli is a single non-glycosylated polypeptide chain containing of 144 amino acids. A fully biologically active molecule, rrIL-3 beta has a molecular mass of 16.3 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
  • Specification
  • Gene Information
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Description : Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific α chain and a common β chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family.
Source : E. coli
Species : Rat
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : ED50 < 5 ng/mL, measured by a cell proliferation assay using NFS-60 cells, corresponding to a specific activity of > 2 × 10^5 units/mg.
Molecular Mass : 16.3 kDa, observed by non-reducing SDS-PAGE.
Protein Length : 144
AA Sequence : MISDRGSDAHHLLRTLDCRTIALEI LVKLPYPQVSGLNNSDDKANLRNST LRRVNLDEFLKSQEEFDSQDTTDIK SKLQKLKCCIPAAASDSVLPGVYNK DLDDFKKKLRFYVIHLKDLQPVSVS RPPQPTSSSDNFRPMTVEC
Endotoxin : < 0.2 EU/μg, determined by LAL method.
Purity : > 95% as analyzed by SDS-PAGE and HPLC.
Storage : Lyophilized recombinant rat Interleukin-3 beta (rrIL-3 beta) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rrIL-3 beta remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
Storage Buffer : Lyophilized after extensive dialysis against PBS.
Reconstitution : Reconstituted in ddH2O at 100 μg/mL.
Gene Name : Il3 interleukin 3 [ Rattus norvegicus (Norway rat) ]
Official Symbol : Il3
Synonyms : Il3; interleukin 3; interleukin-3; IL-3; MCGF; P-cell-stimulating factor; hematopoietic growth factor; mast cell growth factor; multipotential colony-stimulating factor
Gene ID : 24495
mRNA Refseq : NM_031513
Protein Refseq : NP_113701
UniProt ID : F1LS41

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (16)

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What is the difference between IL-3 and GM-CSF In vitro? 04/14/2023

In vitro, IL-3 can induce differentiation and proliferation of myeloid lineage cells and is distinguished from GM-CSF by the relative increase in production of platelets, basophils, and mast cells.

What is the difference in clinical presentation of IL-3 compared to GM-CSF? 04/14/2023

Although effective, IL-3 appears to present no therapeutic advantages compared to G-CSF and GM-CSF, and in direct comparison studies, IL-3 led to a considerably higher rate of adverse events than either of the other cytokines.

Since the structure of IL-3 is different between animals and humans, does it mean that il-3 is not necessary to maintain stable hematopoietic function? 04/14/2023

mice deficient in both bc and IL-3 show the same phenotype as the bc-deficient mice, conclusively demonstrating that, for the most part, the GM-CSF/IL3/IL5 family is not essential for steady-state production of myeloid cells

How does the structure of IL-3 in animals differ from that in humans? 04/14/2023

Unlike in humans, murine IL-3 has a second b chain receptor, bcIL3, which bears 91% homology to the bc chain.

Does loss of cytokine IL-3 affect normal granulocyte, monocyte, and basophil levels? 04/14/2023

Knockout mice were also reported with surprisingly limited phenotypes. Despite the ability of the three cytokines to stimulate hematopoiesis, mice functionally deficient in all three cytokinesdisplay normal circulating levels of granulocytes, monocytes, and basophils.

IL-3 specific receptor subunit CD123 is overexpressed in many hematological malignancies,Is it overexpressed in normal tissues where inflammation occurs? 04/14/2023

non-hematopoietic (e.g., epithelial cells, and fibroblasts) cell subsets can also produce il-3.

When is IL-3 activated by T cells? 04/14/2023

Such as allergic inflammation and parasitic infestation.

Since basophils are the target and source of IL-3, can they regulate the secretion of IL-3 to treat allergic diseases? 04/14/2023

IL-3 can regulate the survival of basophil granulocytes and cytokine production through autocrine, so as to achieve the purpose of treating allergic diseases.

T cell IL-3 expression is specifically regulated by which transcription factor? 04/14/2023

Bhlhe40

Which hematopoietic and non-hematopoietic cell subsets express IL-3? 04/14/2023

E.g., lymphocytes,epithelial cells, and fibroblasts

Are there any completed clinical trials on IL3? 12/20/2021

Yes, there are some completed clinical trials on IL3 results, but the specific results need to refer to the relevant research papers.

What is the effect of IL3 on hematopoietic system? 09/29/2021

This protein can promote stem cell proliferation and differentiation during hematopoietic process, thus increasing the production of different types of blood cells.

Are there any side effects of IL3? 09/24/2019

In general, the IL3 has relatively few side effects, but some unpleasant symptoms such as fever, headache and nausea may occur.

IL3 associated with whether the immune adjustment? 06/28/2019

IL3 can regulate the body's immune response and the function of immune cells, and plays an important role in immune regulation.

What is the solubility and stability of IL3? 05/25/2019

IL3 generally have good solubility and stability, making them easier to handle during drug preparation and storage.

How is the research progress of IL3? 02/27/2019

The study of IL3 is ongoing, including its application in the development of therapeutic drugs, as well as in combination therapy with other immune molecules.

Customer Reviews (3)

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06/20/2022

    I've used many brands, but this one is one of my favorites.

    03/08/2021

      The performance is very stable and the price is very reasonable. I would definitely buy this product again.

      12/19/2020

        I have recommended this brand to my colleagues and friends, and they all praise this product.

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