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Active Recombinant Rat Tnfrsf1a protein(Met1-Ala211), hFc-tagged

Cat.No. : Tnfrsf1a-7449R
Product Overview : Recombinant Rat TNFRSF1A (P22934) (Met1-Ala211) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.
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  • Gene Information
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Source : HEK293
Species : Rat
Tag : C-hFc
Protein length : Met1-Ala211
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of RatTNFα. 2. Immobilized mouse TNFa (80-235) at 10 μg/ml (100 μl/well) can bind Rat TNFRSF1A-Fc, The EC50 of Rat TNFRSF1A-Fc is 0.08-0.20 μg/ml.
Molecular Mass : The recombinant Rat TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 431 amino acids and has a predicted molecular mass of 47.9 kDa. The apparent molecular mass of the protein is approximately 59 kDa in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Tnfrsf1a tumor necrosis factor receptor superfamily, member 1a [ Rattus norvegicus ]
Official Symbol : Tnfrsf1a
Synonyms : TNFRSF1A; tumor necrosis factor receptor superfamily, member 1a; tumor necrosis factor receptor superfamily member 1A; p55; p60; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor receptor 1; tumor necrosis factor receptor type I; Tnfr1; MGC105478;
Gene ID : 25625
mRNA Refseq : NM_013091
Protein Refseq : NP_037223

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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