Active Recombinant Human TNFRSF1A protein, His-tagged
Cat.No. : | TNFRSF1A-3189H |
Product Overview : | Recombinant Human TNFRSF1A protein(NP_001056.1)(Met1-Thr211), fused with His tag, was expressed in HEK293. |
Availability | February 09, 2025 |
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Species : | Human |
Source : | HEK293 |
Tag : | His |
ProteinLength : | Met1-Thr211 |
Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The EC50 of biotinylated human TNFα is 5-12 μg/mL.2. Measured by its ability to inhibit TNFα-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.05-0.4μg/mL in the presence of 0.25 ng/mL recombinant human TNFα. |
Molecular Mass : | The recombinant human TNFRI consists of 201 amino acids after removal of the signal peptide and has a predicted molecular mass of 22.7 kDa. As a result of glycosylation, the apparent molecular mass of rh TNFRI is 30-35 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 92 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name | TNFRSF1A tumor necrosis factor receptor superfamily, member 1A [ Homo sapiens ] |
Official Symbol | TNFRSF1A |
Synonyms | TNFRSF1A; tumor necrosis factor receptor superfamily, member 1A; TNFR1; tumor necrosis factor receptor superfamily member 1A; CD120a; TNF R; TNF R I; TNF R55; TNFAR; TNFR60; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor-alpha receptor; tumor necrosis factor receptor type 1; tumor necrosis factor binding protein 1; tumor necrosis factor receptor 1A isoform beta; FPF; p55; p60; TBP1; TNF-R; p55-R; TNFR55; TNF-R-I; TNF-R55; MGC19588; |
Gene ID | 7132 |
mRNA Refseq | NM_001065 |
Protein Refseq | NP_001056 |
MIM | 191190 |
UniProt ID | P19438 |
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Not For Human Consumption!
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