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E2F1

  • Official Full Name

    E2F transcription factor 1

  • Overview

    The E2F transcription factors are essential for regulation of the cell cycle. Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit. Six members of the E2F family have been identified, and each E2F subunit has a DNA bind
  • Synonyms

    E2F1; E2F transcription factor 1; RBBP3; transcription factor E2F1; RBP3; PBR3; RBAP-1; RBBP-3; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1; E2F-1; RBAP1;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Chicken
  • Human
  • Mouse
  • E.coli
  • HEK293
  • HEK293T
  • In Vitro Cell Free System
  • Insect Cell
  • Mamanlian cells
  • Mammalian Cell
  • Wheat Germ
  • Flag
  • GST
  • His
  • Myc
  • DDK
  • Myc|DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human E2F1-12245H Recombinant Human E2F1, GST-tagged E.coli GST C-term-350a.a.
Human E2F1-3002H Recombinant Human E2F1 Protein, GST-tagged Wheat Germ GST
Human E2F1-1031H Recombinant Human E2F1, His-tagged Insect Cell His
Human E2F1-238H Recombinant Human E2F1, MYC/DDK-tagged HEK293 Myc/DDK
Human E2F1-6743HCL Recombinant Human E2F1 293 Cell Lysate HEK293 N/A
Human E2F1-2016H Recombinant Human E2F1 Protein (Val88-Phe437), N-His tagged E.coli N-His Val88-Phe437
Human E2F1-1406H Recombinant Human E2F1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human E2F1-2015H Recombinant Human E2F1 Protein (Val264-Phe437), N-His tagged E.coli N-His Val264-Phe437
Human E2F1-9882HFL Recombinant Full Length Human E2F1, Flag-tagged Mamanlian cells Flag
Human E2F1-4107HF Recombinant Full Length Human E2F1 Protein, GST-tagged In Vitro Cell Free System GST 437 amino acids
Mouse E2f1-2707M Recombinant Mouse E2f1 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Chicken E2F1-6772C Recombinant Chicken E2F1 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • E2F1 Related Articles
  • E2F1 Related Research Area

What is E2F1 protein?

E2F1 (E2F transcription factor 1) is a protein coding gene which situated on the long arm of chromosome 20 at locus 20q11. This gene encodes a transcription factor called E2F1 which is a member of E2F proteins family. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The E2F1 protein is consisted of 437 amino acids and its molecular mass is approximately 46.9 kDa.

What is the function of E2F1 protein?

The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Among them, E2F1 can promote cells to enter the S-phase (DNA synthesis phase) and participates in cell proliferation, differentiation and apoptosis. In addition to its role in cell cycle regulation, E2F1 is also involved in other biological processes, such as apoptosis and tumorigenesis. For example, some studies have shown that high expression of E2F1 is associated with the development of certain cancers.

E2F1 related Signaling pathways

Signaling pathways involved in E2F1 include, but are not limited to, cell cycle pathways, RAS pathways, PI3K pathways, Myc pathways and p53 pathways. The analysis also showed that CDK4/6-Rb1/E2F1 pathway proteins are directly or indirectly associated with key molecules of these classical signaling pathways. E2F1 has also been found to be an important node molecule in the cell signaling network in cancer models and is involved in transcriptional regulation of some important genes by talking to other signaling pathways, including NF-κB. E2F1 can also be involved in apoptosis by controlling the expression of mitochondria-associated genes involved in oxidative phosphorylation and electron transfer.

E2F1 Related Diseases

The E2F1 gene is regularly upregulated in many tumor samples, especially in prostate cancer, where it interacts most with other proteins as an important regulator. In addition, abnormal expression or mutation of E2F1 can lead to abnormal cell cycles, which can lead to diseases, for example, in the islet cells of diabetic patients, expression levels of E2F1 are significantly elevated. Some studies have suggested that E2F1 also plays an important role in neurodegenerative diseases. For example, in patients with Alzheimer's disease, expression levels of E2F1 are significantly reduced. E2F1 may also be associated with a number of other diseases, such as liver fibrosis, cardiovascular disease, etc.

E2F1-7.jpg

Fig1. Results from database showed that E2F1 is a valuable prognostic biomarker for cancers Survival prognosis curve (A) Bladder Urothelial Carcinoma, (B) Colon adenocarcinoma, (C) Lung adenocarcinoma; K–M plotter of E2F1 in (D) Lung cancer, (E) Ovarian cancer, (F) Breast cancer.

Bioapplications of E2F1

E2F1 could be served as a prognostic biomarker in cancer patients and higher levels of in cancer patients could predict shorter overall survival and disease-free survival. And it could be used as a potential target for cancer treatment. Given its role in many diseases, it can be used to study the pathogenesis of related diseases, so as to develop effective treatment strategies.

High Purity

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Fig2. SDS-PAGE (E2F1-2015H)

Case Study 1: Fenfang Zhou, 2024

E2F1-3.jpg

Fig1. Western blot analysis showing the protein levels of E2F1 after overexpressing DLGAP5 with a dose gradient.

Case Study 2: Namin Duan, 2023

E2F1-4.jpg

Fig2. HCT116 xenografts treated with vehicle, SAHA, or HR488B were isolated to detect expression of E2F1 by Western blot analysis, and β-actin was detected as the endogenous loading control, accordingly.

E2F1-5.jpg

AKT Signaling Pathway from R&D

E2F1-6.jpg

MAPK Signaling Pathway (Mitogen Stimulation Pathway) from R&D

E2F1 involved in several pathways and played different roles in them. We selected most pathways E2F1 participated on our site, such as Activation of BH3-only proteins, Activation of NOXA and translocation to mitochondria, Activation of PUMA and translocation to mitochondria, which may be useful for your reference. Also, other proteins which involved in the same pathway with E2F1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Activation of BH3-only proteinsBCL2L11;AKT3;YWHAQ;E2F1;DYNLL2;CDK5RAP2
Activation of NOXA and translocation to mitochondria
Activation of PUMA and translocation to mitochondria
AdipogenesisMEF2AA;NAMPT;CEBPB;CELF1;EBF1;AHR;MIXL1;DVL1;KLF7A
ApoptosisBAXB;AKT2;PIK3CA;BIRC5A;STK24A;PPP3R2;DNM1L;CFLAR;VIML
Assembly of the pre-replicative complexE2F3;MCM8;GMNN;E2F2;CDT1;E2F1
Association of licensing factors with the pre-replicative complexE2F3;CDT1;GMNN;E2F1;E2F2
Bladder cancerRASSF1;ERBB2;MMP1A;THBS1;RAF1;MMP2;CXCL8;BRAF;ARAF

E2F1 has several biochemical functions, for example, DNA binding, core promoter binding, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by E2F1 itself. We selected most functions E2F1 had, and list some proteins which have the same functions with E2F1. You can find most of the proteins on our site.

Function Related Protein
DNA bindingHER4.2;SMARCA5;POLR3A;HOMEZA;ZNF624;ZFP583;TMEM18;ZFPM1;ZFP668
core promoter bindingSFPI1;INSM1A;AGO2;TFAP2C;SAFB;HDAC1;FOXO3;KDM3A;IRF9
protein bindingPLK3;GPR125;RANGAP1;MC1R;DRD3;KDM4A;TNNI3;CCDC151;C14orf169
protein kinase bindingRNF138;GCSAM;NR4A3;TEX14;SLC12A5;CADPS;PRKAG2;CCNL1A;CAV2
sequence-specific DNA bindingHOXB4A;NKX3;MSXA;HOXD10;UNCX;HOXB13;NKX2-4;LHX1B;PGBD1
transcription factor activity, sequence-specific DNA bindingKDM5B;ZFP287;ZKSCAN4;FOXG1;FEV;GRHL3;E2F3;ZFP354B;CITED2
transcription factor bindingTCF12;EGR1;PARK7;PPP1R13B;MDFIC;INSIG2;FOXA2;APBB3;CCND1

E2F1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with E2F1 here. Most of them are supplied by our site. Hope this information will be useful for your research of E2F1.

RB1

Wu, M; Zhou, JL; et al. E2F1 suppresses cardiac neovascularization by down-regulating VEGF and PlGF expression. CARDIOVASCULAR RESEARCH 104:412-422(2014).
Jiang, DF; Aguiar, RCT; et al. MicroRNA-155 controls RB phosphorylation in normal and malignant B lymphocytes via the noncanonical TGF-beta 1/SMAD5 signaling module. BLOOD 123:86-93(2014).
  • Q&As
  • Reviews

Q&As (5)

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Can E2F1 be used in prognostic assessments for cancer patients? 12/21/2022

E2F1 expression patterns may serve as prognostic indicators, helping predict the likelihood of disease progression and informing treatment strategies.

How does E2F1 contribute to drug resistance in cancer? 10/31/2022

E2F1 overexpression has been linked to drug resistance in cancer cells, presenting a challenge in chemotherapy and necessitating further research for effective solutions.

What other diseases or conditions are associated with E2F1 dysfunction? 11/14/2020

E2F1 dysregulation has been implicated in various diseases beyond cancer, including neurodegenerative disorders and cardiovascular diseases.

Are there any clinical trials targeting E2F1 for therapeutic interventions? 05/15/2017

Clinical trials are investigating the potential of targeting E2F1 in cancer treatment, exploring novel drugs and treatment approaches.

How does E2F1 interact with other proteins in cellular processes? 06/05/2016

E2F1 interacts with several proteins to form complexes that regulate gene expression, influencing cellular processes such as DNA replication and repair.

Customer Reviews (3)

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Reviews
06/20/2022

    The versatility of the E2F1 protein is truly remarkable, as it can be employed across a wide range of applications in my research.

    01/16/2022

      In addition to its outstanding quality, the manufacturer of the E2F1 protein offers excellent technical support that can effectively address any challenges I may encounter.

      03/23/2016

        Their dedicated team of experts is readily available to provide guidance and assistance, ensuring a seamless integration of the E2F1 protein into my experimental setup.

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