Recombinant Human E2F1 Protein (Val88-Phe437), N-His tagged

• Cat.No.: E2F1-2016H
• Product Overview: Recombinant human E2F1 (Val88-Phe437) fused with N-terminal His tag was expressed in E. coli.

Specification

• Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Positively regulates transcription of RRP1B.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 40.93 kDa
• Protein Length: Val88-Phe437
• Purity: >90% as determined by SDS-PAGE
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Lyophilized from PBS, pH 7.4, 0.02% NLS, 1mM EDTA, 4% trehalose, 1% mannitol.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: E2F1 E2F transcription factor 1 [ Homo sapiens (human) ]
• Official Symbol: E2F1
• Synonyms: E2F1; E2F transcription factor 1; RBBP3; transcription factor E2F1; RBP3; PBR3; RBAP-1; RBBP-3; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1; E2F-1; RBAP1
• Gene ID: 1869
• mRNA Refseq: NM_005225
• Protein Refseq: NP_005216
• MIM: 189971
• UniProt ID: Q01094