Recombinant Human E2F1 293 Cell Lysate

• Cat.No.: E2F1-6743HCL

Specification

• Description: Antigen standard for E2F transcription factor 1 (E2F1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: E2F1 E2F transcription factor 1 [ Homo sapiens ]
• Official Symbol: E2F1
• Synonyms: E2F1; E2F transcription factor 1; RBBP3; transcription factor E2F1; RBP3; PBR3; RBAP-1; RBBP-3; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1; E2F-1; RBAP1
• Gene ID: 1869
• mRNA Refseq: NM_005225
• Protein Refseq: NP_005216
• MIM: 189971
• UniProt ID: Q01094
• Chromosome Location: 20q11
• Pathway: Activation of BH3-only proteins, organism-specific biosystem; Activation of NOXA and translocation to mitochondria, organism-specific biosystem; Activation of PUMA and translocation to mitochondria, organism-specific biosystem; Adipogenesis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Assembly of the pre-replicative complex, organism-specific biosystem; Association of licensing factors with the pre-replicative complex, organism-specific biosystem
• Function: DNA binding; DNA binding; core promoter binding; cyclin-dependent protein kinase activity; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription corepressor activity; transcription factor binding

Reviews

06/20/2022

The versatility of the E2F1 protein is truly remarkable, as it can be employed across a wide range of applications in my research.

01/16/2022

In addition to its outstanding quality, the manufacturer of the E2F1 protein offers excellent technical support that can effectively address any challenges I may encounter.

03/23/2016

Their dedicated team of experts is readily available to provide guidance and assistance, ensuring a seamless integration of the E2F1 protein into my experimental setup.

Q&As

Can E2F1 be used in prognostic assessments for cancer patients?

E2F1 expression patterns may serve as prognostic indicators, helping predict the likelihood of disease progression and informing treatment strategies.

How does E2F1 contribute to drug resistance in cancer?

E2F1 overexpression has been linked to drug resistance in cancer cells, presenting a challenge in chemotherapy and necessitating further research for effective solutions.

What other diseases or conditions are associated with E2F1 dysfunction?

E2F1 dysregulation has been implicated in various diseases beyond cancer, including neurodegenerative disorders and cardiovascular diseases.

Are there any clinical trials targeting E2F1 for therapeutic interventions?

Clinical trials are investigating the potential of targeting E2F1 in cancer treatment, exploring novel drugs and treatment approaches.

How does E2F1 interact with other proteins in cellular processes?

E2F1 interacts with several proteins to form complexes that regulate gene expression, influencing cellular processes such as DNA replication and repair.