Recombinant Mouse E2f1 Protein, Myc/DDK-tagged

• Cat.No.: E2f1-2707M
• Product Overview: Purified recombinant protein of mouse full-length E2F transcription factor 1 (E2f1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Positively regulates transcription of RRP1B.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 46.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: E2f1 E2F transcription factor 1 [ Mus musculus (house mouse) ]
• Official Symbol: E2f1
• Synonyms: E2F1; E2F transcription factor 1; transcription factor E2F1; E2F-1; mKIAA4009; KIAA4009
• Gene ID: 13555
• mRNA Refseq: NM_007891
• Protein Refseq: NP_031917
• UniProt ID: Q61501

Reviews

06/20/2022

The versatility of the E2F1 protein is truly remarkable, as it can be employed across a wide range of applications in my research.

01/16/2022

In addition to its outstanding quality, the manufacturer of the E2F1 protein offers excellent technical support that can effectively address any challenges I may encounter.

03/23/2016

Their dedicated team of experts is readily available to provide guidance and assistance, ensuring a seamless integration of the E2F1 protein into my experimental setup.

Q&As

Can E2F1 be used in prognostic assessments for cancer patients?

E2F1 expression patterns may serve as prognostic indicators, helping predict the likelihood of disease progression and informing treatment strategies.

How does E2F1 contribute to drug resistance in cancer?

E2F1 overexpression has been linked to drug resistance in cancer cells, presenting a challenge in chemotherapy and necessitating further research for effective solutions.

What other diseases or conditions are associated with E2F1 dysfunction?

E2F1 dysregulation has been implicated in various diseases beyond cancer, including neurodegenerative disorders and cardiovascular diseases.

Are there any clinical trials targeting E2F1 for therapeutic interventions?

Clinical trials are investigating the potential of targeting E2F1 in cancer treatment, exploring novel drugs and treatment approaches.

How does E2F1 interact with other proteins in cellular processes?

E2F1 interacts with several proteins to form complexes that regulate gene expression, influencing cellular processes such as DNA replication and repair.