Description : |
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
46.7 kDa |
AA Sequence : |
MALAGAPAGGPCAPALEALLGAGAL RLLDSSQIVIISAAQDASAPPAPTG PAAPAAGPCDPDLLLFATPQAPRPT PSAPRPALGRPPVKRRLDLETDHQY LAESSGPARGRGRHPGKGVKSPGEK SRYETSLNLTTKRFLELLSHSADGV VDLNWAAEVLKVQKRRIYDITNVLE GIQLIAKKSKNHIQWLGSHTTVGVG GRLEGLTQDLRQLQESEQQLDHLMN ICTTQLRLLSEDTDSQRLAYVTCQD LRSIADPAEQMVMVIKAPPETQLQA VDSSENFQISLKSKQGPIDVFLCPE ETVGGISPGKTPSQEVTSEEENRAT DSATIVSPPPSSPPSSLTTDPSQSL LSLEQEPLLSRMGSLRAPVDEDRLS PLVAADSLLEHVREDFSGLLPEEFI SLSPPHEALDYHFGLEEGEGIRDLF DCDFGDLTPLDFTRTRPLEQKLISE EDLAANDILDYKDDDDKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |