"Protease" Related Products

TEV Protease Fluorometric Activity Assay Kit

Cat.No.: Kit-2138
Product Overview: TEV Protease Activity assay kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). This assay kit is simple, rapid and can detect TEV Protease activity as low as 50 ng/well in viral lysates and purified protein samples.
Size: 100 assays
Description: TEV Protease (EC:, Tobacco Etch Virus protease) is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-YXaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). TEV Protease has high specificity and great stability and is active over a wide range of temperatures (4-37°C) with an optimal activity at 34°C.
Applications: Detection of TEV Protease activity in viral lysates Determine activity of purified TEV Protease.
Target Species: Viral lysates
Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read entire protocol before performing the assay.TEV Protease Assay Buffer: Add 100 µl of 1 M DTT to the TEV Protease Assay Buffer. Mix well. Store at -20°C. Bring to room temperature before use.TEV Protease (Positive Control): Aliquot and store at -80°C. Avoid repeated freeze/thaw.
Kit Components: TEV Protease Assay Buffer: 25 mlTEV Protease Dilution Buffer: 10 ml1 M DTT: 100 μlTEV Protease (Positive Control): 10 μlTEV Protease Substrate: 100 μl5-FAM Standard (100 µM in DMSO): 100 μl
Detection method: Fluorescence (Ex/Em = 490/560 nm)
Compatible Sample Types: Viral Lysates, purified protein
Features & Benefits: • Simple, rapid assay• Can detect as low as 50 ng/well of TEV Protease activity• Fluorometric Detection

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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