||TNKS1 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the substrate proteins.
||The TNKS1 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) is designed to measure Tankyrase 1 (TNKS1) activity for screening and profiling applications. The key to the TNKS1 Histone Ribosylation Assay (Biotin-labeled NAD+) is the biotinylated substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 384-well plate. Next, the biotinylated TNKS1 substrate is incubated with an assay buffer that contains the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
||Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
||12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
||TNKS1: 2 x 5 µg; -80°C 5 x histone mixture: 2 x 1 ml; -80°C10x Assay Mixture Containing Biotinylated Substrate: 2 x 300 μl; -80°C10x PARP Assay Buffer: 2 x 1 ml; -80°CBlocking buffer: 50 ml; +4°CStreptavidin-HRP: 2 x 100 μ; +4°CHRP chemiluminescent substrate A (translucent bottle): 2 x 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 2 x 6 ml; +4°C 384-well plate: 1; Room Temperature