"TNKS1" Related Products

TNKS1 Histone Ribosylation Colorimetric Assay Kit

Cat.No.: Kit-1883
Size: 96 reactions
Description: The TNKS1 Histone Ribosylation Colorimetric Assay Kit is designed to measure Tankyrase 1 (TNKS1) activity for screening and profiling applications. TNKS1 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the substrate proteins. The key to the TNKS1 Histone Ribosylation Assay is the biotinylated NAD+ substrate. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 96-well plate. Next, the biotinylated NAD+substrate is incubated with an assay buffer that contains the TNKS1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components: TNKS1: 5 µg; -80°C 5 x histone mixture: 1 ml; -80°C10x Assay Mixture Containing Biotinylated Substrate: 300 μl; -80°C10x PARP Assay Buffer: 1 ml; -80°CBlocking buffer: 25 ml; +4°CStreptavidin-HRP: 100 μ; +4°CColorimetric HRP substrate: 10 ml; +4°C Transparent 96-well plate: 1; Room Temperature

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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