"WDR9/BRWD1" Related Products

WDR9/BRWD1 TR-FRET Assay Kit

Cat.No.: Kit-1891
Size: 384 reactions
Description: The WDR9 TR-FRET Assay Kit is designed to measure the inhibition of the binding of WDR9, also known as BRWD1, to its substrate in a homogeneous 384 reaction format. This FRETbased assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, WDR9, substrate, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
Storage: At least 6 months from date of receipt when stored as directed. Be sure to store each component at the proper temperature upon arrival.
Kit Components: WDR9, GST-tag: 10 µg; -80°CBET Bromodomain Ligand: 75 µl; -80°CNon-acetylated Ligand 1: 15 μl; -80°CTb donor: 20 µl; -20°CDye-labeled acceptor: 20 µl; -20°C3x BRD TR-FRET Assay Buffer 1: 4 ml; -20°CWhite Nonbinding low volume microtiter plate: 1; Room temp.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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