Active Recombinant Full Length Human TP53 Protein, Flag tagged

• Cat.No.: TP53-01HFL
• Product Overview: Recombinant p53 (TP53) protein was expressed in a baculovirus expression system as the full length protein (accession number NP_000537.3) with an N-terminal FLAG tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag
• Protein Length: 1-393 aa
• Description: p53 (TP53) (Tumor Protein P53), also known as Phosphoprotein P53, Transformation-Related Protein 53 or Antigen NY-CO-13, is a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. It involves in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. p53 induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1, thus participating in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. It is required for apoptosis induction, which seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF, p53 also is involved in activating oxidative stress-induced necrosis, which is largely independent of transcription.
• Molecular Mass: 44.9 kDa
• Optimal pH: 0
• Isoelectric Point: 0
• Purity: ≥ 95%
• Application: Recombinant p53 (TP53) protein is suitable for use in the study of protein-protein interaction, in vitro transcription assay, binding assay.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Storage Buffer: Recombinant p53 (TP53) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP.

Gene Information

• Gene Name: TP53 tumor protein p53 [ Homo sapiens (human) ]
• Official Symbol: TP53
• Synonyms: TP53; tumor protein p53; cellular tumor antigen p53; LFS1; Li Fraumeni syndrome; p53; antigen NY-CO-13; mutant p53 protein; phosphoprotein p53; p53 tumor suppressor; truncated p53 protein; tumor suppressor TP53; transformation-related protein 53; P53; TRP53; FLJ92943
• Gene ID: 7157
• mRNA Refseq: NM_000546
• Protein Refseq: NP_000537
• MIM: 191170
• UniProt ID: P04637