Species : |
Human |
Source : |
Insect Cells |
Tag : |
Flag |
Protein Length : |
1-393 aa |
Description : |
p53 (TP53) (Tumor Protein P53), also known as Phosphoprotein P53, Transformation-Related Protein 53 or Antigen NY-CO-13, is a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. It involves in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. p53 induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1, thus participating in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. It is required for apoptosis induction, which seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF, p53 also is involved in activating oxidative stress-induced necrosis, which is largely independent of transcription. |
Molecular Mass : |
44.9 kDa |
Purity : |
≥ 95% |
Application : |
Recombinant p53 (TP53) protein is suitable for use in the study of protein-protein interaction, in vitro transcription assay, binding assay. |
Storage : |
Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
Storage Buffer : |
Recombinant p53 (TP53) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP. |