| Description : |
This gene encodes a secreted ligand of the glial cell line-derived neurotrophic factor (GDNF) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein signals through the RET receptor and GFR alpha 3 coreceptor, and supports the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. This protein has also been shown to promote tumor growth, metastasis, and drug resistance in mammary carcinoma. |
| Source : |
E. coli |
| Species : |
Human |
| Bio-activity : |
Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is 4-16 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha -3/GDNF R alpha -3 Fc Chimera at 1 μg/mL (100 μL/well) can bind Recombinant Human Artemin with an apparent Kd <1 nM. |
| Molecular Mass : |
24.2 kDa |
| Product-Related Proteins : |
108-220 |
| Endotoxin : |
< 0.1 EU/μg of the protein as determined by the LAL method |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
At -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Storage Buffer : |
PBS |
