Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Join NIH Research Festival Biotech Vendor Exhibits 2024 | Sep. 25th, 2024

Active Recombinant Human CCL11 Protein (74 aa)

Cat.No. : CCL11-129C
Product Overview : Recombinant Human CCL11 Protein without tag was expressed in E. coli.
  • Specification
  • Gene Information
  • Related Products
  • Download
Description : CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the aminoterminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.
Source : E. coli
Species : Human
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : Fully biologically active when compared to standard. Measured by its ability to chemoattract mouse CCR3 transfected BaF3 mouse pro-B cells.The ED50 for this effect is typically 1-5 ng/mL, corresponding to a Specific Activity of >2 × 10^5 IU/mg.
Molecular Mass : 8.3 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
Protein length : 74
AA Sequence : GPASVPTTCCFNLANRKIPLQRLES YRRITSGKCPQKAVIFKTKLAKDIC ADPKKKWVQDSMKYLDQKSPTPKP
Endotoxin : Less than 1 EU/mg of rHuEotaxin/CCL11 as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analyses.
Storage : This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : CCL11 chemokine (C-C motif) ligand 11 [ Homo sapiens ]
Official Symbol : CCL11
Synonyms : CCL11; chemokine (C-C motif) ligand 11; SCYA11, small inducible cytokine subfamily A (Cys Cys), member 11 (eotaxin); eotaxin; eotaxin 1; MGC22554; eotaxin-1; C-C motif chemokine 11; small-inducible cytokine A11; eosinophil chemotactic protein; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin); SCYA11;
Gene ID : 6356
mRNA Refseq : NM_002986
Protein Refseq : NP_002977
MIM : 601156
UniProt ID : P51671

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Reviews
  • Q&As

Customer Reviews (0)

Write a review

Q&As (0)

Ask a question

Ask a Question for All CCL11 Products

Required fields are marked with *

My Review for All CCL11 Products

Required fields are marked with *

0

Inquiry Basket

cartIcon
logo

FOLLOW US

Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /
  • Service lnquiry:

Stay Updated on the Latest Bioscience Trends