Active Recombinant Human CX3CL1 protein, His/Avi-tagged, Biotinylated
Cat.No. : | CX3CL1-051H |
Product Overview : | Biotinylated Recombinant Human CX3CL1(Gln25-Thr338) protein, fused to His/Avi tag at the C-terminus, was expressed in HEK293 cells . |
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- Gene Information
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Description : | Fractalkine, also known as CX3C motif chemokine 1, CX3CL1, neurotactin and small-inducible cytokine D1, is the only member of the CX3C subfamily of the chemokine superfamily (1). Mature human Fractalkine consists of an N-terminal chemokine domain with a CX3C motif and a mucin-like stalk region in the extracellular domain (ECD), a transmembrane segment and a short cytoplasmic domain (1, 2). The soluble form of Fractalkine is generated via ADAM10 and ADAM17 cleavage (1). Within the ECD, human Fractalkine shares 59% amino acid sequence identity with both mouse and rat Fractalkine. Fractalkine exists as both a membrane-bound adhesion molecule and as a soluble proinflammatory chemoattractant and anti-inflammatory neuroprotective agent (1-3). The expression of CX3CL1 is higher in spinal metastases from kidney cancer (4). The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes (5). Most of the functions of CX3CL1 are exerted through the CX#CL1/CX3CR1 axis which has the therapeutic prospect (5, 6). Our Avi-tag Biotinylated Recombinant Fractalkine features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity. |
Source : | HEK293 cells |
Species : | Human |
Tag : | His/Avi |
Predicted N Terminal : | Gln25, inferred from enzymatic pyroglutamate treatment revealing His26 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CX3CR1. The ED50 for this effect is 20-100 ng/mL. Measured by its binding ability in a functional ELISA. |
Molecular Mass : | 88-103 kDa, under reducing conditions |
Protein length : | Gln25-Thr338 |
Endotoxin : | <1.0 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Binding Activity, Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 500 μg/mL in PBS. |
Gene Name : | CX3CL1 chemokine (C-X3-C motif) ligand 1 [ Homo sapiens ] |
Official Symbol : | CX3CL1 |
Synonyms : | CX3CL1; chemokine (C-X3-C motif) ligand 1; SCYD1, small inducible cytokine subfamily D (Cys X3 Cys), member 1 (fractalkine, neurotactin); fractalkine; ABCD 3; C3Xkine; CXC3; CXC3C; neurotactin; NTN; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); NTT; SCYD1; ABCD-3; |
Gene ID : | 6376 |
mRNA Refseq : | NM_002996 |
Protein Refseq : | NP_002987 |
MIM : | 601880 |
UniProt ID : | P78423 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionCX3CL1 is involved in pain modulation, and its dysregulation is associated with neuropathic pain conditions. Understanding its role may lead to the development of novel analgesic therapies.
Yes, several clinical trials are investigating the therapeutic potential of targeting CX3CL1 in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
CX3CL1 contributes to inflammation in cardiovascular diseases and affects vascular function. Studying its role helps in understanding the pathophysiology of conditions like atherosclerosis.
CX3CL1 acts as a chemoattractant, facilitating the recruitment and activation of immune cells at the site of inflammation, playing a pivotal role in immune response regulation.
Researchers are exploring CX3CL1 as a potential biomarker for diseases such as rheumatoid arthritis and certain cancers, aiming to develop diagnostic tools and monitor disease progression.
Customer Reviews (3)
Write a reviewthe CX3CL1 protein's compatibility with various experimental techniques further supports its usability.
One of the standout features of the CX3CL1 protein is the excellent technical support provided by the manufacturer.
Whether I need to study protein-protein interactions, protein localization, or protein folding, the CX3CL1 protein consistently delivers precise and meaningful data.
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