Recombinant Human chemokine (C-X3-C motif) ligand 1, His-tagged

• Cat.No.: CX3CL1-114H
• Product Overview: Recombinant human CX3CL1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW=10.9 kDa (97aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CX3CL1, also known as fractalkine. CX3CL1 has a unique CX3C cysteine motif near the amino terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin like stalk. It is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Also, CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Form: Liquid. In Phosphate buffered saline (pH7.4), 10% glycerol.
• Purity: > 90% by SDS-PAGE.
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQHHGVTKCN ITCSKMTSKI PVALLIHYQQ NQASCGKRAI ILETRQHRLF CADPKEQWVK DAMQHLDRQA AALTRNG.
• Storage: Can be stored at +4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CX3CL1 chemokine (C-X3-C motif) ligand 1 [ Homo sapiens ]
• Synonyms: CX3CL1; chemokine (C-X3-C motif) ligand 1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); C-X3-C motif chemokine 1; Neurotactin; Small-inducible cytokine D1; Processed fractalkine
• Gene ID: 6376
• mRNA Refseq: NM_002996
• Protein Refseq: NP_002987
• MIM: 601880
• UniProt ID: P78423
• Chromosome Location: 16q13
• Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction; Signaling by GPCR
• Function: chemokine activity