EFNA3-196H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant human EphrinA3 (NP_004943.1) (Met 1-Ser 213) with the C-terminal propeptide removed was fused with the Fc region of human IgG1 at the C-terminus.
Source :
HEK293
Species :
Human
Tag :
Fc
Predicted N Terminal :
Gln 23
Form :
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity :
Measured by its binding ability in a functional ELISA. Immobilized mouse EphA6 at 1 μg/ml (100 μl/well) can bind human EphrinA3 / Fc Chimera. The EC50 of human EphrinA3 is 299.2 ng/mL.
Molecular Mass :
The recombinant human EphrinA3/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 429 amino acids and has a predicted molecular mass of 48 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphrinA3/Fc monomer is approximately 60-65 kDa due to glycosylation.
Endotoxin :
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity :
>97 % as determined by SDS-PAGE.
Stability :
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage :
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.