Active Recombinant Human EFNA3 protein, Fc-His tagged

• Cat.No.: EFNA3-77H
• Product Overview: Active Recombinant Human EFNA3 protein(NP_004943.1) (Met 1-Ser 213) with the C-terminal propeptide removed was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-213 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EphA6-His at 10 μg/mL (100 μl/well) can bind human EphrinA3-Fch, The EC50 of human EphrinA3-Fch is 36.0-85.0 ng/mL.
• Molecular Mass: The recombinant human EphrinA3/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 439 amino acids and has a predicted molecular mass of 49.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphrinA3/Fc monomer is approximately 60-65 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EFNA3 ephrin-A3 [ Homo sapiens ]
• Official Symbol: EFNA3
• Synonyms: EFNA3; ephrin-A3; EPLG3; Ehk1 L; LERK3; EFL-2; LERK-3; EHK1 ligand; ligand of eph-related kinase 3; eph-related receptor tyrosine kinase ligand 3; EFL2; Ehk1-L
• Gene ID: 1944
• mRNA Refseq: NM_004952
• Protein Refseq: NP_004943
• MIM: 601381
• UniProt ID: P52797