Active Recombinant Human EIF2C2, His-tagged
| Cat.No. : | EIF2C2-2180H |
| Product Overview : | Recombinant Human AGO2(Met 1-Ala 859), fussed with His tag at N-terminal, was expressed in Baculovirus-Insect Cells. |
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| Description : | Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines. |
| Source : | Baculovirus-Insect Cells |
| Species : | Human |
| Tag : | His |
| Predicted N Terminal : | Met |
| Form : | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4,10% glycerol,2mM DTT1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements. |
| Bio-activity : | Human AGO2 can bind Let-7a RNA and cleave target RNA (21nt). |
| Molecular Mass : | The recombinant human AGO2 consists of 877 amino acids and predicts a molecular mass of 99 kDa as estimated by SDS-PAGE under reducing conditions. |
| Protein length : | Met 1-Ala 859 |
| Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : | > 85 % as determined by SDS-PAGE. |
| Stability : | Samples are stable for up to twelve months from date of receipt at -70 centigrade. |
| Storage : | Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| Publication : |
| Gene Name : | AGO2 argonaute RISC catalytic component 2 [ Homo sapiens ] |
| Official Symbol : | AGO2 |
| Synonyms : | Q10; EIF2C2; protein argonaute-2; CTA-204B4.6; PAZ Piwi domain protein; PPD; argonaute 2; argonaute2; eIF-2C 2; eIF2C 2; eukaryotic translation initiation factor 2C, 2; hAgo2; protein slicer |
| Gene ID : | 27161 |
| mRNA Refseq : | NM_012154 |
| Protein Refseq : | NP_036286 |
| MIM : | 606229 |
| UniProt ID : | Q9UKV8 |
| Chromosome Location : | 8q24 |
| Pathway : | Adaptive Immune System, organism-specific biosystem; Ca2+ pathway, organism-specific biosystem; Downstream signal transduction, organism-specific biosystem |
| Function : | RNA 7-methylguanosine cap binding; RNA polymerase II core binding; core promoter binding |
Products Types
| ◆ Recombinant Protein | ||
| AGO2-526H | Recombinant Human AGO2 Protein, MYC/DDK-tagged | +Inquiry |
| AGO2-1728H | Recombinant Human AGO2 Protein (517-818 aa), His-tagged | +Inquiry |
| Ago2-1556M | Recombinant Mouse Ago2 Protein, Myc/DDK-tagged | +Inquiry |
| AGO2-2180H | Recombinant Human AGO2, His-tagged | +Inquiry |
| AGO2-3126H | Recombinant Human AGO2 protein, His-tagged | +Inquiry |
| ◆ Lysates | ||
| AGO2-714HCL | Recombinant Human AGO2 cell lysate | +Inquiry |
| AGO2-576MCL | Recombinant Mouse AGO2 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (21)
Ask a questionResearch explores how AGO2 guides small RNAs to complementary target mRNA sequences.
Research explores AGO2’s role in guiding small RNAs to their target sequences.
Yes, AGO2 is part of the antiviral RNA interference pathway.
Yes, AGO2’s activity leads to the suppression or degradation of target mRNAs.
Research investigates AGO2’s connection to chromatin remodeling complexes.
AGO2 dysfunction is linked to disorders involving impaired gene regulation.
Yes, AGO2 is essential for normal development and cellular processes.
AGO2 plays a central role in mediating RNA silencing and post-transcriptional regulation.
AGO2 helps control gene expression by degrading or inhibiting mRNA translation.
Yes, AGO2’s function is important for maintaining stem cell populations.
Yes, AGO2’s activity can be regulated in response to cellular stress.
Yes, AGO2’s role in gene regulation is relevant in cancer studies.
Yes, AGO2’s role extends to epigenetic regulation through RNA-directed DNA methylation.
Yes, AGO2’s function is linked to post-transcriptional regulation of circadian genes.
Yes, AGO2’s activity can influence heterochromatin formation and genome stability.
AGO2’s activity leads to translational repression through mRNA cleavage or inhibition.
Yes, AGO2 is involved in suppressing transposable element activity.
AGO2 interacts with small RNAs, including microRNAs and small interfering RNAs.
Research explores AGO2’s contribution to targeting RNAs for exosome-mediated decay.
Research investigates AGO2’s role in regulating germline development and fertility.
AGO2’s activity impacts neuronal development and synaptic plasticity.
Customer Reviews (5)
Write a reviewThe high purity of the protein product facilitated accurate quantification of target molecules. -
Well-defined gel electrophoresis bands were indicative of the protein product’s pure form. -
Incorporating the product led to a noticeable increase in target protein expression levels. -
Precise results from immunoprecipitation assays were attributed to the protein product’s purity. -
Consistency across experiments made the protein product a reliable research tool. -
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