Active Recombinant Human AGO2 protein, His-tagged

• Cat.No.: AGO2-2180H
• Product Overview: Active Recombinant Human AGO2 protein(NP_036286.2) (Met 1-Ala 859) was expressed in Insect Cells with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-859 aa
• Form: Lyophilized from sterile 20mM Tris,500mM NaCl,10% glycerol, pH7.4,5% Trehalose,5%mannitol.
• Bio-activity: Human AGO2 can bind Let-7a RNA and cleave target RNA (21nt).
• Molecular Mass: The recombinant human AGO2 consists of 877 amino acids and predicts a molecular mass of 99 kDa as estimated by SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Probing the nanoparticle–AGO2 interaction for enhanced gene knockdown (2018)