Active Recombinant Mouse Erbb4 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Erbb4-7416MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Erbb4 protein (Q61527) (Met1-Leu652) is produced by HEK293 Cells expression system. This protein is fused with a His tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Leu652， 638
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse ERBB4-His at 10 μg/mL (100 μL/well) can bind biotinylated human NRG1, The EC50 of biotinylated human NRG1 is 0.55-1.29 μg/mL.
• Molecular Mass: The recombinant mouse ERBB4 comprises 638 amino acids and has a predicted molecular mass of 71.5 kDa. The apparent molecular mass of the protein is approximately 113 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/mL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Shipping: Shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: Erbb4 v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) [ Mus musculus ]
• Official Symbol: Erbb4
• Synonyms: ERBB4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); Her4; c-erbB-4
• Gene ID: 13869
• mRNA Refseq: NM_010154
• Protein Refseq: NP_034284
• UniProt ID: Q61527