| Description : |
Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Recombinant FGF-basic 154 is the full length FGF-basic protein encoded by the human FGF-2 gene. FGF-basic 154 is the most popular tissue culture product at Shenandoah Biotechnology, Inc. There are no detectable differences in biological activity between FGF-basic 154 and the truncated FGF-basic 147 proteins. |
| Source : |
E. coli |
| Species : |
Human |
| Bio-activity : |
3T3 Proliferation, ≤5 ng/mL; ≥2.0 x 10^5 units/mg |
| Molecular Mass : |
Monomer, 17.3 kDa (155 aa) |
| AA Sequence : |
MAAGSITTLPALPEDGGSGAFPPGH FKDPKRLYCKNGGFFLRIHPDGRVD GVREKSDPHIKLQLQAEERGVVSIK GVCANRYLAMKEDGRLLASKCVTDE CFFFERLESNNYNTYRSRKYTSWYV ALKRTGQYKLGSKTGPGQKAILFLP MSAKS |
| Endotoxin : |
≤1 EUs/μg, Kinetic LAL |
| Purity : |
≥95%, Reducing and Non-Reducing SDS PAGE |
| Stability : |
12 months from date of receipt when stored at -20 to -80 centigrade as supplied.
1 month when stored at 4 centigrade after reconstituting as directed.
3 months when stored at -20 to -80 centigrade after reconstituting as directed. |
| Storage : |
Storage Prior to Reconstitution: -20 centigrade |
| Storage Buffer : |
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 8.0 |
| Reconstitution : |
Sterile water at 0.1 mg/mL |
| Shipping : |
Room temperature |
| Instructions : |
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 centigrade and avoid repeat freeze thaws. |
