Active Recombinant Human FGF2 Protein

• Cat.No.: FGF2-82H
• Product Overview: Recombinant Human FGF2 Protein without tag was expressed in E. coli.

Specification

• Description: Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Recombinant FGF-basic 154 is the full length FGF-basic protein encoded by the human FGF-2 gene. FGF-basic 154 is the most popular tissue culture product at Shenandoah Biotechnology, Inc. There are no detectable differences in biological activity between FGF-basic 154 and the truncated FGF-basic 147 proteins.
• Source: E. coli
• Species: Human
• Bio-activity: 3T3 Proliferation, ≤5 ng/mL; ≥2.0 x 10^5 units/mg
• Molecular Mass: Monomer, 17.3 kDa (155 aa)
• AA Sequence: MAAGSITTLPALPEDGGSGAFPPGH FKDPKRLYCKNGGFFLRIHPDGRVD GVREKSDPHIKLQLQAEERGVVSIK GVCANRYLAMKEDGRLLASKCVTDE CFFFERLESNNYNTYRSRKYTSWYV ALKRTGQYKLGSKTGPGQKAILFLP MSAKS
• Endotoxin: ≤1 EUs/μg, Kinetic LAL
• Purity: ≥95%, Reducing and Non-Reducing SDS PAGE
• Stability: 12 months from date of receipt when stored at -20 to -80 centigrade as supplied.
  1 month when stored at 4 centigrade after reconstituting as directed.
  3 months when stored at -20 to -80 centigrade after reconstituting as directed.
• Storage: Storage Prior to Reconstitution: -20 centigrade
• Storage Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 8.0
• Reconstitution: Sterile water at 0.1 mg/mL
• Shipping: Room temperature
• Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 centigrade and avoid repeat freeze thaws.

Gene Information

• Gene Name: FGF2 fibroblast growth factor 2 (basic) [ Homo sapiens (human) ]
• Official Symbol: FGF2
• Synonyms: FGF2; fibroblast growth factor 2 (basic); FGFB; fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF; BFGF; FGF-2; HBGF-2
• Gene ID: 2247
• mRNA Refseq: NM_002006
• Protein Refseq: NP_001997
• MIM: 134920
• UniProt ID: P09038

Reviews

07/14/2022

The technical expert communicated with me in time to understand the progress of my experiment and gave me suggestions for improvement.

02/04/2022

The purchased protein is suitable for WB experiments and I am satisfied with the results.

Q&As

What is the biological function of FGF2?

FGF2 promotes cell migration and angiogenesis and regulates bone homeostatic balance.

Where is FGF2 mainly expressed?

FGF2 is widely expressed in the nervous system.

In which tissues of sheep is FGF2 primarily expressed?

FGF2 is mainly expressed in tissues such as mammary glands and ovaries of sheep.

What protein does FGF2 share high homology with?

FGF1 has a high degree of homology with FGF2.

What diseases is FGF2 involved in?

FGF2 is involved in diseases such as cardiovascular disease, breast disease and bone homeostasis.

How does FGF2 regulate bone homeostasis?

FGF2 negatively regulates bone homeostasis.