|Product Overview:||Recombinant bovine Fibroblast Growth Factor-Basic containing 155 amino acids without tag was expressed in E. coli.|
|Description:||FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.|
|Form:||Sterile Filtered White lyophilized (freeze-dried) powder.|
|Molecular Mass:||17.25 kDa|
|Identity:||Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1 mg/mL) solution. This value is calculated by the PC GENE computer analysis program of protein sequences.
2. Analysis by RP-HPLC, using a calibrated solution of FGF-2 Bovine as a Reference Standard.
|N-terminal Sequence Analysis:||The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.|
|Purity:||Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
|Storage:||Lyophilized Fibroblast Growth Factor 2 Bovine although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution FGF-b Bovine Recombinant should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade. Please prevent freeze-thaw cycles.|
|Concentration:||The FGF-b Bovine was lyophilized from a concentrated (1 mg/mL) sterile solution containing 1% HSA.|
|Reconstitution:||It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-2 Bovine Recombinant in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.|
|Shipping:||At room temperature|
|Gene Name:||FGF2 fibroblast growth factor 2 [ Bos taurus (cattle) ]|
|Synonyms:||FGF2; fibroblast growth factor 2; BFGF; FGF-2; HBGF-2; fibroblast growth factor 2; basic fibroblast growth factor; fibroblast growth factor 2 (basic); heparin-binding growth factor 2|