Active Recombinant Human FGF21 Protein

• Cat.No.: FGF21-85H
• Product Overview: Recombinant Human FGF21 Protein without tag was expressed in E. coli.

Specification

• Description: Fibroblast growth factor 21 (FGF-21) is an endocrine hormone that regulates energy homeostasis and exerts cardioprotective functions during heart injury. FGF-21 is expressed in the liver, pancreas, heart, and adipose tissues. FGF-21 signaling is activated through the FGFR1c receptor and β-Klotho co-receptor. FGF-21 is an important regulator of glucose uptake and reduces cell apoptosis under stress conditions.
• Source: E. coli
• Species: Human
• Bio-activity: 3T3 proliferation with β-klotho and heparin, ED50≤500 ng/mL (≥2.0 x 10^3 unit/mg)
• Molecular Mass: Monomer, 19.5 kDa (182 aa)
• AA Sequence: MHPIPDSSPLLQFGGQVRQRYLYTD DAQQTEAHLEIREDGTVGGAADQSP ESLLQLKALKPGVIQILGVKTSRFL CQRPDGALYGSLHFDPEACSFRELL LEDGYNVYQSEAHGLPLHLPGNKSP HRDPAPRGPARFLPLPGLPPALPEP PGILAPQPPDVGSSDPLSMVGPSQG RSPSYAS
• Endotoxin: ≤1 EUs/μg, Kinetic LAL
• Purity: ≥95%, Reducing and Non-Reducing SDS PAGE
• Stability: 12 months from date of receipt when stored at -20 to -80 centigrade as supplied.
  1 month when stored at 4 centigrade after reconstituting as directed.
  3 months when stored at -20 to -80 centigrade after reconstituting as directed.
• Storage: Storage Prior to Reconstitution: -20 centigrade
• Storage Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5
• Reconstitution: Sterile water at 0.1 mg/mL
• Shipping: Room temperature
• Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 centigrade and avoid repeat freeze thaws.

Gene Information

• Gene Name: FGF21 fibroblast growth factor 21 [ Homo sapiens (human) ]
• Official Symbol: FGF21
• Synonyms: FGF21; fibroblast growth factor 21; FGF-21
• Gene ID: 26291
• mRNA Refseq: NM_019113
• Protein Refseq: NP_061986
• MIM: 609436
• UniProt ID: Q9NSA1

Reviews

01/04/2022

The manufacturer's history of producing high-quality products contributed to our trust in this protein.

06/27/2021

Collaboration with other research groups was made easier by the product's standardized use in the scientific community.

03/03/2016

The product's role in promoting collaboration and teamwork positively impacted the quality of our research.

Q&As

How is FGF21 expression regulated in adipose tissue?

FGF21 expression in adipose tissue is regulated by various factors, including peroxisome proliferator-activated receptor gamma (PPARγ) activation.

What are the effects of FGF21 on lipid metabolism?

FGF21 influences lipid metabolism by promoting the utilization of fatty acids for energy, contributing to the regulation of lipid homeostasis.

Can FGF21 be a biomarker for metabolic health?

FGF21 levels are being explored as potential biomarkers for metabolic health, reflecting conditions such as insulin resistance and metabolic syndrome.

Is FGF21 involved in the regulation of insulin sensitivity?

Yes, FGF21 plays a role in improving insulin sensitivity, making it a potential target for interventions in insulin resistance-associated conditions.

Does FGF21 have roles in energy expenditure?

Yes, FGF21 influences energy expenditure by promoting thermogenesis in brown adipose tissue, contributing to overall metabolic regulation.

Are there gender-specific differences in FGF21 levels?

Gender-specific differences in FGF21 levels have been reported, with some studies suggesting higher circulating FGF21 concentrations in females.

What signaling pathways does FGF21 activate?

FGF21 activates signaling pathways such as the AMP-activated protein kinase (AMPK) pathway, which contributes to its metabolic effects.