Active Recombinant Human GZMB protein, His-tagged

• Cat.No.: GZMB-01H
• Product Overview: Recombinant human Granzyme B protein Gly19-Tyr247 was expressed in NS0, with a C-terminal 10-His tag.

Specification

• Description: This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis
• Source: NS0
• Species: Human
• Tag: His
• Form: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB).
• Molecular Mass: Predicted Molecular Mass: 27 kDa
  SDS-PAGE: 30-40 kDa, reducing conditions
• N-terminal Sequence Analysis: Gly19
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: GZMB granzyme B [ Homo sapiens (human) ]
• Official Symbol: GZMB
• Synonyms: C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GranzymeB; GRB; GrzB; GZMB; HLP; SECT
• Gene ID: 3002
• mRNA Refseq: NM_004131.4
• Protein Refseq: NP_004122.2
• MIM: 123910
• UniProt ID: P10144