Recombinant Murine Granzyme B

• Cat.No.: Gzmb-942M
• Product Overview: Recombinant murine Granzyme B produced inInsect cellsis a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B.

Specification

• Cat. No.: Gzmb-942M
• Description: Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues.
• Source: Insect cells.
• Purity: > 98% by SDS-PAGE.
• Endotoxin Level: < 0.1 ng per μg of Granzyme-B.
• Stabilizer: None.
• Formulation: Lyophilized.
• Biological Activity: Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25℃, is greater than 750 nM/min per μg of enzyme.
• Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -20℃. Reconstituted Granzyme-B is stable for at least 3 months when stored in working aliquots with a carrier protein at -20℃. Avoid repeated freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: Gzmb granzyme B [ Mus musculus ]
• Synonyms: Gzmb; granzyme B; GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11; EC 3.4.21.79
• Gene ID: 14939
• mRNA Refseq: NM_013542
• Protein Refseq: NP_038570
• UniProt ID: P04187
• Chromosome Location: 14 20.5 cM
• Pathway: Allograft rejection; Autoimmune thyroid disease; Graft-versus-host disease; Natural killer cell mediated cytotoxicity; Type I diabetes mellitus
• Function: hydrolase activity; peptidase activity; protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Reviews

11/01/2020

I am delighted to express my complete satisfaction with the quality of GZMB protein, as it precisely suits my experimental requirements.

08/29/2017

Their knowledgeable team has been readily available to address any concerns or queries, providing effective solutions and guidance whenever needed.

07/28/2017

I have full confidence in the GZMB protein and the exceptional support provided by the manufacturer, making it my top recommendation for researchers seeking a high-quality protein for their experimental needs.

Q&As

What are some potential future applications of GZMB in medicine?

GZMB may be used in the development of targeted therapies for viral infections and various immune-related disorders.

How is GZMB protein clinically relevant in cancer treatment?

GZMB can be used in cancer immunotherapy to target and eliminate cancer cells.

What are the challenges in using GZMB in cancer immunotherapy?

One challenge is to ensure that GZMB selectively targets cancer cells without causing harm to healthy cells.

What types of cancer can be targeted using GZMB-based therapies?

GZMB-based therapies can be used against various types of cancer, including leukemia, lymphoma, and solid tumors.

Can GZMB be used in autoimmune disease treatment?

GZMB's role in autoimmune diseases is complex. It can contribute to tissue damage in some autoimmune conditions, but its use in treatment is not common.